Antibody Engineering and Optimization
Our antibody engineering group provides both stand-alone and integrated service packages starting from antibody generation to the delivery of humanized and optimized monoclonal or bispecific antibodies. Our stand-alone antibody engineering services include hybridoma sequencing, phage library construction, high-throughput panning, mouse antibody humanization, antibody affinity maturation, Fc engineering and bispecific antibody generation.
- Our humanization strategies include both Best Fit and Framework Library approaches designed to minimize the risk of affinity and functional activity loss caused by the traditional CDR grafting method.
- We provide affinity maturation services using random and parsimonious mutagenesis on selected CDRs to optimize antibody affinity and cross-reactivity to orthologous target antigens.
- We enhance the antibody in vivo stability and ADCC / CDC potency by Fc engineering using site-specific mutation and glycosylation modification.
- Bispecific antibodies are generated either from de novo discovery using transgenic rats carrying common light chain or by engineering a pair of mono-specific antibodies provided from you or from WuXi’s mAb discovery program.