WuXi Biologics
Offering End-to-End Solutions
In Vitro Lead Characterization
WuXi Biologics provides assay development services and offers comprehensive biologics screening and characterization throughout the lead drug candidate selection process. Our biology team has extensive discovery experience with various targets and therapeutic modalities including but not limited to mAbs, bsAbs, multispecific antibodies, ADCs, fusion proteins, and immunocytokines.
Binding
Affinity by SPR (Surface Plasmon Resonance)
Bispecific antibody simultaneous binding
Related SPR service
Epitope Binning and Mapping
Peptide mapping, domain mapping and alanine scanning mutagenesis services are available to assist customers in obtaining antigen epitope information, including linear and conformational epitopes. We have successfully completed more than 30 epitope mapping projects for the benefit of our clients’ antibody drug design & discovery projects.
Peptide mapping (linear epitope)
Domain mapping (domain epitope)
Alanine Scanning Mutagenesis (both linear and conformational epitopes)
Functional Assays
Biochemical Assays
Test item | Analysis methods |
Antigen-antibody binding assay |
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Competition Assay |
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Cross species/paralogue binding (protein or cell-based, ELISA, FACS) | |
Signal Transduction (Phosphorylation) |
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cAMP signaling assay | |
Enzymatic assays | |
Receptor occupancy |
Cellular Assays
Test item | Analysis methods |
Reporter gene assay (RGA) |
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Translocation imaging assay | |
PBMC activation |
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T/NK/B cell activation and expansion | |
Tumor dependent immune system activation |
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Fc dependent killing and phagocytosis assay |
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Fc independent killing |
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Cytotoxicity assay (CellTiter-Glo) | |
Bystander killing assay | |
Proliferation assay (CFSE/BrdU/CellTiter-Glo) | |
Cell panel screening (Viability/proliferation) | |
3D culture assay (Proliferation, killing) | |
Cell cycle analysis | |
Apoptosis assay |
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Monocyte survival | |
Differentiation and polarization |
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Antibody Internalization |
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Cell Migration/Invasion Assay |
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Surface marker profiling | |
Target expression profiling on resting or activated cells | |
Cytokine/chemokine profiling |
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Ex vivo tumor and TIL profiling | |
CAT-T cell activation |
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CAR-T cell killing | |
Allogeneic Mixed Lymphocytes Reaction (MLR) | |
Antigen specific MLR/T cell recall assay (CMV, TT) | |
T cell exhaustion | |
Regulatory T cell assay |
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Hemagglutination | |
Metabolic diseases | |
CNS |
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Infectious disease |
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In vitro safety evaluation |
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Developability evaluation |
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