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Protein Sciences

Uniting Drug Development Expertise with Antibody & Protein Production Services

Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) Binding Assays

SPR and BLI Analysis

SPR and BLI binding assays are optical techniques used to monitor and analyze real-time interactions between biomolecules, providing valuable insights into binding kinetics, affinities, and more.

SRP and BLI binding assays that monitor and analyze real-time interactions between biomolecules.

Applications of BLI/SPR

SRP and BLI binding assays can be applied to compounds, nucleic acid, proteins, lipid and virus.

Surface Plasmon Resonance (SPR)

SPR is an optical method used to monitor real-time molecular interactions. This occurs when plane-polarized light interacts with a metal film under specific reflection conditions (total internal reflection).

Benefits of SPR:

 

  • Versatile: Capable of detecting most types of molecules
  • Wide range: Analyze bound molecules at μM to fM levels

SPR is a real-time, label-free method of sensitive detection of molecules interactions.

SPR (Biacore)-based Binding Assay Service Details

Activity-Part Description Duration Request A Quote
Assay development Single assay condition, single sensor type, affinity/kinetics profile for the target, 1 interaction included 1-2 weeks Request A Quote
Measurement Based on established assay conditions, measure the affinity/kinetics for 1 interaction 1-2 weeks
Screening Single concentration, response level and off-rate will be provided (if applicable) 1-2 weeks

Case Study: SPR’s Diverse Analysis Capabilities

 

This case study demonstrates the core capability offered by SPR for determining binding kinetics, affinities, epitope binning, and concentration analysis.

Figure A: Presents a distinct kinetic binding pattern resulting from the interaction between an antibody and antigen. This data set shows a strong representative example of an analyte with high affinity to its target.

Showcase an analyte with high affinity to its target with SPR assays.

Showcase the effectiveness of epitope binning through SPR.

Figure B: Shows the effectiveness of epitope binning through SPR. A secondary antibody (blue) was introduced after the formation of an antibody-antigen complex. A competing antibody was tested in parallel (red). This figure indicates that the two antibodies possess distinct epitopes that don’t overlap.

Figures C & D: Shows the concentration analysis of an analyte with and without calibration.

Showcase the concentration analysis of an analyte with and without calibration.

Bio-Layer Interferometry (BLI)

BLI is a label-free optical method that provides real-time measurements of changes in optical thickness at a biosensor’s surface. It achieves this by analyzing the interference patterns created when a light beam reflects off the biosensor’s surface and the biomolecular layer on top of it. The strength of the signal directly relates to the size of the molecule bound to the sensors.

BLI (octet)-based Binding Assay Service Details

Activity-Part Description Duration Request A Quote
Assay development Single assay condition, single sensor type, affinity/kinetics profile for the target, 1 interaction included 1-2 weeks Request A Quote
Measurement Based on established assay conditions, measure the affinity/kinetics for 1 interaction 1-2 weeks
Screening Single concentration, response level and off-rate will be provided (if applicable)

<150 Interaction:

1-2 weeks

>150 Interaction:

TBD

Figures A & B: Demonstration of the fast and accurate titer measurements obtained with BLI, with pre-setup calibration curve. 

Showcase the fast and accurate titer measurements obtained with biolayer interferometry (BLI).

Showcase the fast and accurate titer measurements obtained with biolayer interferometry (BLI).

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