Our naïve human library was constructed from PBMCs isolated from healthy donors. Antibody V-genes were amplified from mRNA using V-gene family specific primers and cloned into a phage display vector in scFv format. The library consists more than 1011 individual transformants with ~90% containing full ScFv transcripts. ~80% of clones show detectable scFv expression into the periplasm.
- Constructed in-house from individual PBMC&CBMC samples and commercial mixed donor cDNAs (high diversity)
- More than 1011 transformants representing nearly all human antibody V-gene repertoire
- Pre-selected in vivo, “truly human”, no synthetic or artificial sequences
- Each sub-library is systematically QC’d to assure library quality