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Antibody Generation

WuXi Biologics can provide antibody generation through traditional hybridoma approach and fully human antibody generation using transgenic animals. WuXi Biologics has successfully delivered 150+ therapeutic antibody discovery projects via our hybridoma platform. We have extensive experiences with GPCR (G Protein-Coupled Receptor)  & other difficult targets.

 


 

Process and Timeline:

 

Process and Timeline

 


 

Key Features:

 

  • Transgenic mice/rats for fully human antibody generation
  • Multiple immunization strategies (DNA, peptide, protein, cell or combinations thereof) to generate the best-fit to the targets 
  • Proprietary immunization protocols to increase immune responses
  • High fusion efficiency through electrofusion 
  • Semi-solid method for subcloning
  • High throughput protein-based and cell-based screening platforms. Five high-throughput flow cytometers and seven Biacore instruments to support screening and affinity ranking.
  • Functional screen using hybridoma supernatants to increase chances of isolating functional leads.

WuXi Biologics offers high-quality phage display library construction and customized library selection and screening services.

 

WUXI BIO logo

WuXi Biologics has constructed human naïve libraries from 50+ donors with more than 1011 transformants representing nearly all human antibody V-gene repertoire:

 

 

  • Naïve library panning/screening: 2-3 months.
  • Immune library construction and screening: 3-4 months

 

Process and Timeline:

 

Antibody generation through phage display

 

Key Features:

 

  • In-house ready to use human naïve or synthetic libraries
  • Immune (custom) libraries: human, mouse, rat, llama, alpaca, common light chain
  • Liquid-phase, solid-phase and cell-based panning
  • Epitope guided and Avi-tag capture selections enable isolation of functionally active antibodies

 


WuXi Biologics offers high-quality alpaca/llama immune library construction and customized library selection and screening services. WuXi Biologics has constructed alpaca naïve libraries from 50+ alpaca donors with more than 10<sup>11</sup> transformants:

 

  • Naive library panning/screening: 2-3 months.
  • Immune library construction and screening: 3-4 months                                      

 

Process and Timeline:

 

VHH (single domain antibody) generation

 

Key Features:

 

  • Liquid-phase, solid-phase and cell-based panning
  • In-house ready to use alpaca naïve libraries
  • Proprietary primer sets to achieve increased coverage of alpaca/llama VHH gene repertoire