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Protein Sciences

Uniting Drug Development Expertise with Antibody & Protein Production Services

LC-MS Services

Liquid Chromatography-Mass Spectrometry (LC-MS) Analysis

Our cutting-edge LC-MS services enable precise molecular analysis, providing you with invaluable insights into your biologics.

 

  • Detailed characterization of bispecific antibodies (bsAbs) by intact mass analysis
  • HTP intact mass analysis for protein confirmation and quick glycan analysis (96-well plate)
  • Detailed peptide mapping for sequence coverage, post-translational modifications (PTMs), and sequence variant analysis
  • Disulfide mapping analysis to determine unknown disulfide linkage
  • N-glycan analysis by LC-Fluorescence-MS/MS for complex glycosylation

Our LC-MS services enable precise molecular analysis, providing you with invaluable insights into your biologics.

Intact Mass LC-MS Service Details:

(For Protein Samples)

Service Item Description Quantity Test Window Request A Quote
Intact mass LC-MS
(simple protein & vanilla antibody）

1. Sample and sequence requirement: sample amount >20 μg, concentration >0.5 mg/ml1

 

2. Sample preparation: reduce/non-reduce; degly/without degly option provided

 

3. Deliverables:

 

  1. Confirm protein sequence: main peak ID
  2. Check sequence homogeneity: >10% (MS intensity%) minor impurity ID
 1-10 samples <1 week Request A Quote 
11-20 samples 1-2 weeks
>20 samples 2 weeks
Intact mass LC-MS
(protein with more than 2 glycosylation sites & bi-,tri-specific and fusion protein）

1. Sample and sequence requirement: sample amount >50 μg, concentration >0.5 mg/ml1

 

2. Sample prep: reduce/non-reduce option provided

 

  1. Unique deglycosylation protocol to remove complex glycan and reduce heterogenicity if necessary

 

3. Deliverables:

 

  1. Confirm protein sequence: main peak ID
  2. Check sequence homogeneity: >10% (MS intensity%) minor impurity ID
 1-10 samples <1 week
11-20 samples 1-2 weeks
>20 samples 2 weeks

Intact mass LC-MS after IdeS digestion （Quick N-glycan）
  1. Sample and sequence requirement: sample amount >50 ug, concentration >0.5mg/ml1
  2. Sample prep: reduce intact after IdeS digestion;
  3. Deliverables:
  • Confirm protein sequence: main peak ID
  • Report major glycosylation forms and percentage Now
 1-10 samples After <1W
1-10 samples    <1W
11-20 samples   1-2W

High-throughput protein ID by intact mass LC-MS

(96 well plate antibody confirmation)

 

1. Sample and sequence requirement: sample amount (96 well plate format) >20 ug, concentration >0.5 mg/ml1

 

2. Sample prep: reduce/non-reduce; degly/nondegly option (generally deglycosylation not needed)

 

3. Deliverables:

 

  1. Confirm protein sequence: main peak ID
  2. Check sequence homogeneity: >50% (MS intensity%) minor impurity ID
 >20 samples 2 weeks
50-96 samples <1 week
MS spectrum and peak list only

1. Sample and sequence requirement: sample amount (96 well plate format) >20 μg, concentration >0.5 mg/ml1

 

2. Sample prep: reduce/non-reduce; degly/without degly option provided

 

3. Deliverables: MS spectrum and peak list only

 No limit 2 days

 

Note:
1. For sample amount and concentration lower than specified requirement, please consult with BD
2. For other special needs, please consult with BD

Peptide Map LC-MS Service Details:

(For Protein Samples)

Service Item Description Quantity Test Window Request A Quote
Peptide mass analysis
(Mass ID confirm）

1. Sample and sequence requirement: sample amount >30 μg, concentration >0.5 mg/ml1

 

2. Deliverables:

 

  1. Confirm protein sequence: sequence coverage map
 1-3 samples 2 weeks Request A Quote 
3-5 samples 2-3 weeks
>5 samples 3-4 weeks
Peptide mass analysis
(Based on software）

1. Sample and sequence requirement: sample amount >50 μg, concentration >0.5 mg/ml1

 

2. Deliverables:

 

  1. Confirm protein sequence: sequence coverage map
  2. Software-based identification for PTM, SV and fragment et al analysis.
 1-3 samples 2-3 weeks
3-5 samples 3-4 weeks
>5 samples 4-5 weeks

Peptide mass analysis
(Based on software & manual check）

 

1. Sample and sequence requirement: sample amount >50 μg, concentration >0.5 mg/ml1

 

2. Deliverables:

 

  1. Confirm protein sequence: sequence coverage map
  2. Check sequence homogeneity: software-based identification for PTM, SV and fragment et al analysis.
  3. Check sequence homogeneity: manual check for all components with user defined intensity threshold (>1%) for detailed PTM, SV and fragment et al analysis
 1-3 samples TBD
3-5 samples TBD
>5 samples TBD

 

Note:
1. For sample amount and concentration lower than specified requirement, please consult with BD
2. For other special needs, please consult with BD

Disulfide LC-MS Service Details:

(For Protein Samples)

Service Item Description Quantity Test Window Request A Quote
Disulfide mapping analysis
(To confirm known disulfide linkage）

1. Sample and sequence requirement: sample amount >50 μg, concentration >0.5 mg/ml1

 

2. Deliverables:

 

  1. Confirm known disulfide linkage information
  2. Report free cysteine amount and any mis-linked disulfide bond with >5% abundance2

 

 1-3 samples 2-3 weeks Request A Quote
3-5 samples 3-4 weeks
>5 samples 4-5 weeks
Disulfide mapping analysis
(To determine unknown disulfide linkage）
  1. Sample and sequence requirement: sample amount >50 μg, concentration >0.5 mg/ml1

 

2. Deliverables: determine unknown disulfide linkage for every cystein

 1 sample 3 weeks

 

Note:
1. For sample amount and concentration lower than specified requirement, please consult with BD
2. For user-defined threshold and any other special needs, please consult with BD

N-glycan LC-MS Service Details:

(For Protein Samples)

Service Item Description Quantity Test Window Request A Quote
N-glycan analysis

1. Sample and sequence requirement: sample amount >100 μg, concentration >0.5mg/ml1

 

2. Deliverables:

 

  1. Report any glycan relative abundance >1% based on fluorescence2
  2. Report glycan ID based on mass spec results

 

 1-5 samples 2 weeks   Request A Quote  
>5 samples 2-3 weeks

 

Note:
1. For sample amount and concentration lower than specified requirement, please consult with BD
2. For user-defined threshold and any other special needs, please consult with BD

LC-MS Based Protein Quantification Service Details:

Service Item Description Quantity Test Window Request A Quote
LC/MS-based protein quantification Pilot testing for feasibility evaluation and experimental protocol set up (including 5 sample testing) 1 sample TBD Request A Quote  
Protein quantification without enrichment step 1-10 samples 2 weeks
10-30 samples 2 weeks
>30 samples 2 weeks
Protein quantification with affinity enrichment step 1-10 samples 2 weeks
10-30 samples 2 weeks
>30 samples 2 weeks

 

Note: Intact mass analysis

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