The production of bispecific antibodies presents certain challenges due to the formation of various byproducts resulting from chain mispairing, homodimer formation, and incomplete assembly. Our Premium Bispecific antibody service is capable of generating high-quality bsAbs, ranging from milligrams to grams, in just 4-6 weeks. Having produced over 3,000 different bsAbs, we have accumulated extensive expertise in this field.
During the expression stage, we undertake construct optimization and chain ratio studies to maximize expression levels of the desired target. In the purification stage, a tailored approach is essential, as the byproducts for each specific bsAb differ. To address this challenge, we employ intact mass spectrometry analysis as a powerful tool to identify and monitor these byproducts during the purification process. This information guides us in selecting the most effective purification steps for pooling and polishing. Subsequently, we scale up the process as needed to produce the desired quantity of the final product.