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LC-MS Services

Intact Mass Analysis, Peptide Mapping, and Glycan Analysis for Biologics Drug Discovery and Optimization

 

Our LC-MS services provide precise, high-throughput protein characterization to help uncover the complexity and heterogeneity of protein therapeutics in early drug discovery. With capabilities in Intact Mass analysis, peptide mapping analysis, disulfide mapping analysis, glycan analysis, and proteomics/PK studies, our LC-MS platform enables identification of post-translational modifications (PTMs), sequence variants, and other critical quality attributes that impact product quality, safety, and efficacy. Backed by robust workflows for both routine and high-throughput studies, these LC-MS protein quantification and characterization services deliver high-quality data across a broad range of biologics, including monoclonal antibodies, bispecific antibodies, and antibody-drug conjugates (ADCs), helping teams make faster, more confident decisions in early biologics drug discovery and optimization.

 

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LC-MS analysis platform used for biologics characterization, supporting intact mass analysis, peptide mapping services, n-glycan analysis, and proteomics/PK study workflows.

Our LC-MS Services Include:

 

  • Intact Mass Analysis: Confirms molecular mass, identifies bispecific antibody byproducts to guide purification workflows, and performs quick glycan checks.
  • Peptide Mapping Analysis: Provides detailed sequence information and identifies expected or unexpected PTMs and mutations.
  • Disulfide Mapping Analysis: Determines disulfide linkage patterns and measures free thiol content.
  • Glycan Analysis: Characterizes glycan location, composition, and occupancy.
  • PK Assessment: Mass Spectrometry-based protein identification and quantification to support in vitro and in vivo PK analysis.

 

 

LC-MS services workflow for biologics drug discovery and optimization, covering intact mass analysis, peptide mapping services, disulfide mapping, glycan analysis, and PK assessment.

 

Intact Mass Analysis Peptide Mapping Analysis Disulfide Mapping Analysis Glycan Analysis PK Assessment
  • Molecular weight confirmation (R/NR) with high-throughput options
  • IdeS digestion for N-glycan analysis
  • PTM identification and monitoring
  • Glycan analysis
  • Sequence variant analysis
  • Disulfide linkage with software & manual check
  • Free thiol content
  • N-glycan analysis by Intact Mass
  • Releasing N-glycan analysis
  • N-Glycan profiling by peptide mapping
  • In vitro: Average DAR, total antibody, PTMs
  • In vivo: Total antibody, average DAR

 

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Case Study #1: High-Throughput Intact Mass Analysis for Rapid Biologics Characterization

 

This case study highlights a high-throughput LC-MS analysis workflow for rapid biologics characterization of monoclonal antibodies, bispecific antibodies, fragment-based antibodies, and other recombinant proteins. With flexible sample preparation approaches such as reduced/non-reduced, subunit generation, and deglycosylation, combined with 2D LC systems, automated data acquisition, and data processing, this Intact Mass Spectrometry workflow supports rapid molecular mass confirmation, heterogeneity identification, and streamlined reporting for early discovery studies.

 

Diagram of a high-throughput intact protein mass spectrometry analysis workflow for biologics, including sample preparation methods, 2D LC instrumentation, automated data processing, and chromatogram outputs for antibody characterization.

 

Figure 1: High-throughput Intact Mass analysis workflow for biologics characterization, showing sample preparation options, 2D UPLC and TOF-MS setup, and automated data processing for rapid mass confirmation and heterogeneity assessment.

 

Case Study #2: Peptide Mapping Analysis Reveals Modification Sites (Oxidation, Dioxidation, Kynurenine) in CDR Region

 

This case study demonstrates how peptide mapping analysis can identify modification hotspots in CDR regions under oxidative stress conditions. The LC-MS workflow detected oxidation, dioxidation, and kynurenine sites after 2 days of oxidative stress treatment. The study also correlated these PTMs with ELISA binding results, showing how peptide mapping services support deeper PTM monitoring and early risk evaluation in biologics discovery.

 

Figure showing peptide mapping analysis results for an antibody under oxidative stress, revaling several modification sites in CDR region.

 

Figure 2: Peptide mapping analysis of an antibody under oxidative, deamidation, and isoAsp stress conditions identified CDR modification sites, including oxidation, dioxidation, and kynurenine, and linked PTMs to ELISA binding activity to confirm the modifications.

 

Get PTM Insights with Peptide Mapping Services

 

 

Case Study #3: Glycan Analysis by LC-MS/MS Identifies Hybrid N-Glycan Structures With and Without Acetylation

 

This case study demonstrates how N-glycan analysis by LC-MS/MS can identify hybrid N-glycan structures with and without acetylation. Using detailed Mass Spec fragmentation patterns, the workflow supported structural assignment of glycan composition and acetylation status, enabling clear N-glycan profiling for biologics characterization.

 

Figure showing LC-MS/MS glycan analysis spectra for a hybrid N-glycan with and without acetylation, highlighting the need for N-glycan structure identification.

 

Figure 3: LC-MS/MS glycan analysis identified a hybrid N-glycan with and without acetylation by comparing fragmentation spectra, supporting detailed N-glycan characterization in biologics.

 

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Case Study #4: LC-MS Protein Quantification of Total Antibody and Average DAR for ADC PK Assessment

 

This case study demonstrates how an LC-MS/MS method can quantify total antibody concentration and average drug-to-antibody ratio (DAR) in biological matrix samples. The workflow integrates immunoprecipitation, protein precipitation, enzyme digestion, and payload release to measure total antibody, conjugated payload, and free payload content. The study highlights how Mass Spectrometry analysis supports robust ADC characterization and enables accurate PK assessment during therapeutic ADC discovery.

 

LC-MS/MS method for ADC PK assessment, illustrating workflows for quantifying total antibody, conjugated payload, free payload content, and average DAR over time in biological matrix samples.

 

Figure 4: LC-MS/MS workflow for ADC PK assessment showing quantification of total antibody and average DAR through immunoprecipitation, protein precipitation, enzyme digestion, enzyme release, and data analysis in biological matrices.

 

Discover How LC-MS/MS Services Support Your Project

 

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