The baculovirus–insect cell expression system is a powerful and versatile platform for the production of a wide range of proteins. This platform offers several unique advantages for recombinant protein expression , such as post-translational modifications, higher yield for secreted protein, and proved solubility and relatively high cell culture density, which render it an excellent choice for the expression of intracellular proteins, protein complexes, kinases, membrane protein and viral proteins. A wide variety of commonly used insect cell lines, such as Sf9, and High Five, are available at WuXi Biologics. By utilizing optimized expression vectors , host and high-titer virus packaging technology, WuXi Biologic can effectively increase protein expression levels and deliver highly quality proteins.
Case Study: Small Scale Protein Kinase Production
Escherichia coli (E. coli) is a popular host option for recombinant protein expression because of its high yield, low cost, and ease of scaling . Thus, bacterial expression systems play an important role in both laboratory investigations and industrial-scale production.
WuXi Biologics have extensive experience with difficult proteins in E. coli expression, both for soluble proteins and inclusion bodies refolding, and provide customers with high-quality recombinant proteins from research to GMP.
E.coli Platform Features
Case Study: E.coli Expression Optimization
E.Coli Scaleup Feasibility
High-density Escherichia coli fermentation in bioreactor can achieve much more biomass by nutrient feeding, sufficient oxygen supplementation along with stable pH control, resulting in higher yield (up to several grams of proteins per liter) of protein expression than shake flask cultivation. Fermentation conditions are highly scalable and reproducible with reducing risk and delays when the process applies to commercial manufacturing. The short manufacturing times ensure a cost-effective production approach for both soluble protein and inclusion body production.
E.coli Scaleup Service Details:
Case Study: Large Scale Production of an Enzyme for Bioconjugation