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CRO Services

Drug Development Expertise Empowering Research Services for Biologics

Phage & Yeast Display

Phage and Yeast Display Libraries for High-Affinity Antibody Discovery

WuXi Biologics offers high-quality phage and yeast display library construction services, supported by tailored library selection and screening methods. Our diverse library platforms have enabled over 200 monoclonal antibody discovery projects, including those targeting challenging molecules, such as G-protein coupled receptors (GPCRs), ion channels, multi-subunit complexes, and small-molecule haptens.

 

Key Features of Phage & Yeast Display Libraries:

  • Comprehensive human scFv native library, human Fab synthetic library, VHH native library and humanized VHH synthetic library
  • Custom libraries derived from multiple species, including mouse, rat, alpaca, llama, camel, rabbit, and chicken
  • Flexible liquid-phase, solid-phase, and cell-based phage panning techniques
  • Targeted epitope-guided and native conformation-captured selection methods
  • Native & synthetic library delivered in 1-2 months; immune library completed within 2-3 months

Phage & Yeast Display Service Details:

Service Items Description Turnaround Time Request a Quote
Native or Synthetic Phage Display Libraries (scFv, Fab, VHH) 1. Human scFv native library: 50+ donors with more than 1011 transformants covering diverse human antibody V-gene repertoire
2. Human Fab synthetic library: 80 dominant germline pairs of heavy and light chains with more than 1012 transformants
3. Camelid native library: 100+ camelid donors with over 5 × 1011 transformants
4. Humanized VHH synthetic library: Design based on identified stable scaffolds with more than 1011 transformants
5. Library constructed from individual donor with validated high quality		 4-8 weeks Request A Quote 
Immunization & Phage/Yeast Display Libraries (VHH, scFv, Fab) 1. Multiple immunization strategies (DNA, mRNA-LNP, peptide, protein, cell, or combinations) tailored for optimal target response
2. Proprietary immunization protocols
3. Custom antigen design
4. Engineered yeast strain with high expression and display level		 16-20 weeks

Case Study #1: Discovery of High-Affinity VHH Antibodies Targeting G-Protein Coupled Receptors (GPCRs)

This case study showcases the successful discovery of VHH antibodies targeting GPCRs from immunized alpacas. Through advanced technologies, we identified high-affinity lead candidates with enhanced binding specificity and potency.

 

Figure A: An overview of the display workflow, including alpaca immunization, phage library construction and cell-based panning, binding validation, and lead candidate identification.

Figure B: Binding affinity analysis using FACS for positive tumor cell line, demonstrating improved EC50 for antibodies produced by our display library (red curves) compared to other benchmark candidates (blue curve).

Case Study #2: Humanized VHH Synthetic Library for High-Quality VHH Antibody Discovery

This case study highlights the robustness of our humanized VHH synthetic library for discovery of high-affinity VHH candidates.

 

Figure A: Our humanized VHH synthetic library was designed and developed based on stable scaffolds. We obtained high-purity display library through single-step Protein A purification, as confirmed by SDS-PAGE. The library also demonstrates enhanced developability with an average Tm value exceeding 60 °C.

Figure B: A VHH lead candidate was identified from the humanized VHH synthetic library, exhibiting improved binding profile to human target X comparable to that of the benchmark antibody. Also, this lead demonstrated effective internalization, optimal yield, and enhanced thermostability.

Case Study #3: Discovery of High-Affinity Antibodies Against a Multi-Pass Transmembrane Protein from Human Native scFv Library

Our human native scFv library was employed to discover high-affinity antibodies targeting a multi-pass transmembrane protein. The identified antibodies exhibited superior specificity and potency, eliminating the need for affinity maturation in ADC therapeutics development.

 

Figure A: The identified antibody demonstrated an enhanced specific binding positive rate (red curve) compared to the benchmark antibody (blue curve). Additionally, these leads candidates exhibited enhanced internalization and efficient in vivo tumor cell killing, positioning them as ideal candidates for ADC development.

 

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