Lead Optimization

WuXi Biologics offers extensive lead assessment and optimization services for antibody therapeutics such as monoclonal (mAb), bispecific (bsAb), and multispecific antibodies and other biologics. Our team of experts work with you to establish the best path in optimizing your molecule to its target and for its intended purpose.

Humanization
Affinity Maturation
Fc Engineering
PTM assessment and optimization

WuXi Biologics offers antibody humanization using best fit approach combined with structure modeling for back-mutation design. 150+ humanization projects completed with >98% success rate.

Key features:

8-10 weeks turnaround time
In parallel PTM removal

lead optimization-Humanization

WuXi Biologics offers antibody affinity maturation through our well-established and highly-reliable parsimonious mutagenesis methodology. We can also offer phage library-based block-mutagenesis approach for affinity maturation of antibodies to cell-surface targets.

Key Features :

4 months turnaround time
Mimics somatic hyper-mutations in natural antibody
Affinity improvement (koff): 10 – 100 folds (sub-nanomolar affinities)
Low risk of epitope shift
Simultaneous removal of PTM risks and engineering of pH-sensitivity

WuXi Biologics offers engineering of antibodies with different Fc variants

Related services:

Binding affinity test to Fc receptors, FcRn, C1q
ADCC/CDC assays

Sequence analysis
PTM sites removal in parallel with other antibody optimization tasks, such as humanization or affinity maturation